Using our proprietary technology we are capable of selecting full-length, fully human antibodies expressed in mammalian cells that bind to your target of interest with properties favorable for therapeutic development.
Benefits of our process include:
• Selection of antibodies with desired activity, binding to specific and diverse epitopes.
• Our libraries are not limited by self tolerance, allowing selection of antibodies with desired species cross-reactivity including human, mouse, monkey or other.
• Candidate heavy and light chains are close to fully human germline, reducing the potential for immunogenicity.
• Campaigns can be designed to use a common light chain resulting in the opportunity to engineer bi-specific therapeutics in different expression formats.
• Selection of antibodies expressed in mammalian cells with built in manufacturability and favorable biophysical properties ideally suited for downstream development. Our antibodies are better producers intrinsically owing to the fact that efficient and stable mammalian cell expression is required for selection. We believe they are comparable or superior to successful commercial antibody therapeutics in terms of solubility, expression, and biophysical characteristics.
We have a broad range of capabilities and expertise that integrate with and complement our selection campaigns using standard and custom assays where desired. This includes in vitro and in vivo assays. We adhere to best practices and highest standards of quality and reporting so that you can be confident in the performance of your antibodies. Our specificity and functional assays include:
• Use of blocking reagents, specificity reagents during selection
• ELISA on positive and control antigens
• Flow Cytometry assays on native antigens expressed on various cell line panels
• IHC for lead antibodies
• Species cross-reactivity
• Epitope coverage
• Affinity improvement
• Functional assays such as receptor binding, competition, downstream function, etc.
• Relative production assessment
• Size Exclusion Chromatography to assess monomer formation
• Differential Scanning Fluorimetry for stability prediction
• Cross-interaction chromatography (CIC) for solubility