Vaccinex constructs separate and modular heavy and light chains libraries. This gives us the flexibility to employ optimization strategies that meet selection needs. Consider the following three examples.
Example 1: Mouse to Human Conversion. If you have a favorite mouse antibody based on specificity and function, we can easily use the mouse heavy or light chain to search for a complementing human chain in a two-step process that results in the isolation of a fully human antibody with comparable specificity and function.
Example 2: Affinity Improvement. Antibodies that are candidates for affinity improvement can be easily introduced into a screen where single-source (heavy or light) diversities are higher and the probability of finding a higher-affinity heavy and light chain combination is greater. We use this technique frequently to find sub-nanomolar affinity antibodies.
Example 3: Creation of Bispecific Molecules. One solution for the manufacture of bispecific antibodies employs a common light chain shared by both antibodies. Because our libraries are modular, Vaccinex is able to successfully select antibodies based on the use of a fixed or static light chain across a wide variety of targets without the time–consuming step of remaking the libraries. In this way, we are able to deliver two antibodies with different heavy chains that pair with the same light chain.