Antigen expression on the cell surface

• Vaccina cDNA library created from antigen expressing cells
• cDNA’s encoded in the vaccinia genome are expressed as though native to the infected cell.
• Host quality control machinery ensure appropriate post-translational modification as well as assembly with any co-receptors or auxiliary proteins depending on cell type chosen.
• Vaccinia virus has a wide host range to support appropriate presentation.

• Every cell expresses one cDNA
• Single color FACS for binding
• Cell sorting for precise control over selection
• Recover virus by simple freeze/thaw

Our Vaccinia Virus technology can identify target proteins using recombinant libraries and FACS. Our strategy offers the following advantages:

• Expression of recombinant proteins in mammalian cells.
• Large cDNA library size to cover millions of potential targets.
• Capability to make custom cDNA libraries from sources known to bind the molecule of interest.
• Ability to accommodate various forms of binding proteins (full length IgG, scFv, Fab, peptide, scaffold, etc.)
• Fast timelines due to reiterative rounds of sorting.