Antigen expression on the cell surface
- Vaccina cDNA library created from antigen expressing cells
- cDNA’s encoded in the vaccinia genome are expressed as though native to the infected cell.
- Host quality control machinery ensure appropriate post-translational modification as well as assembly with any co-receptors or auxiliary proteins depending on cell type chosen.
- Vaccinia virus has a wide host range to support appropriate presentation.
- Every cell expresses one cDNA
- Single color FACS for binding
- Cell sorting for precise control over selection
- Recover virus by simple freeze/thaw
Our Vaccinia Virus technology can identify target proteins using recombinant libraries and FACS. Our strategy offers the following advantages:
- Expression of recombinant proteins in mammalian cells.
- Large cDNA library size to cover millions of potential targets.
- Capability to make custom cDNA libraries from sources known to bind the molecule of interest.
- Ability to accommodate various forms of binding proteins (full length IgG, scFv, Fab, peptide, scaffold, etc.)
- Fast timelines due to reiterative rounds of sorting.