Immunization
Antigen Virions can be used to immunize mice or other animals to generate titers against complex membrane targets.
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Injections do not require adjuvant, and can be used alone or in conjunction with other immunogens like mRNA or cell lines to boost response.
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Since the two forms of poxvirus are antigenically distinct, immunizing with one strain and selections on the other stain minimizes background.
Mouse Sera Titer for Claudin18.2
Balb/c mice were immunized three times with MVA-Claudin18.2 antigen virions. Sera was tested for Claudin18.2 specificity by flow cytometry on Claudin18.2 versus Claudin 18.1 stable cell lines.
Antibody Selection
IgG mRNA isolated from immunized mouse spleen, lymph nodes, or bone marrow
Phage library generation
FPV coupled to magnetic beads for phage panning
Target specific antibodies
Once sufficient titer is achieved, an immunized phage library can be generated for panning on antigen coupled to magnetic beads.
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Bead coupling can be done by direct or indirect methods. Antigen virions can be biotinylated for use.
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Phage panning can also be performed by coating antigen virions to ELISA plates.
Discovery Services
Vaccinex also has a proprietary antibody cDNA library for phage display or mammalian display derived from a combination of synthetic and native sources.
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As an Antibody Discovery CRO, we have a full suite of technologies to facilitate drug discovery at any stage.Â
Target
ProteinÂ
Optimization
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Target ID
Expression
Antigen Virus
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Protein Production
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Stable Cell Line Generation
Discovery
Immunization
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Phage Display
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Mammalian Display
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Hybridoma
Function
Assay Development
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Flow Cytometry
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ELISA
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Optimization
Affinity Improvement
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Humanization