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Image by Stephen Andrews

Pepinemab Oncology

What is Immunotherapy for Cancer?

The immune system is highly effective at attacking and removing any threats to our bodies from infection. This system is tightly regulated to stop after the infection is cleared in order to prevent unwanted effects like autoimmunity.

 

Tumors often manipulate this system to inhibit immune cells by over-expressing the "stop" signal proteins or by preventing immune cells from entering the tumor at all.

Immunotherapy treatments target immunosuppressive proteins like PD-1 and CTLA4 that tumors use to escape. These treatments have greatly improved outcomes for some cancer patients, however many tumors remain resistant due to exclusion of immune cells that could have anti-tumor effects.

SEMA4D in Cancer

Immunohistochemisty images of tumors illustrating SEMA4D localization to the outer edge. Top row shows control treated and bottom row is treated with anti-SEMA4D. Dendritic cells are shown in red and macrophages are shown in green.  CD8+ T cells are shown in red.  Treatment with anti-SEMA4D shows neutralization of the SEMA4D gradient, migration of inflammatory DCs into the tumor and an increase in pro-inflammatory APC cells in the tumor.  The treatment also shows and increase in CD8+ T cell infiltration and activity.

Semaphorin 4D (SEMA4D) is broadly expressed in many human cancers, and its expression correlates with invasive human disease. Preclinical studies have demonstrated that high concentrations of SEMA4D expressed at the invasive margin of tumors can immobilize immune cells and restrict infiltration into tumor. Antibodies against SEMA4D neutralize this barrier and “open the gates” of the tumor to the immune system. This facilitates anti-tumor immune responses and results in reduced tumor burden in animals.

First-in-class Immunotherapy for Cancer

Pepinemab is a novel monoclonal antibody that blocks the activity of SEMA4D. 

Preclinical and early clinical data in melanoma suggest that antibody blockade of SEMA4D increases the number and maturity of immune cell aggregates called Tertiary Lymphoid Structures (TLS) within the tumor microenvironment.  These TLS are correlated with greater tumor regression by functioning as an anti-tumor immune hub.

Immunohistochemistry illustration of a tertiary lymphoid structure where immune cells are colored in pink, blue, orange and red. Tumor is indicated by gray and black.

Immunohistochemistry showing a Tertiary Lymphoid Structure (TLS). Each color represents a different immune cell type.

Note: Pepinemab (VX15/2503) is an investigational drug currently in clinical studies. It has not been demonstrated to be safe and effective for any disease indication. There is no guarantee that pepinemab (VX15/2503) will be approved for the treatment of any disease by the U.S. Food and Drug Administration or by any other health authority worldwide.

Stacked Open Magazines

SITC Nov 7, 2025

Neoadjuvant pepinemab enhances immune checkpoint blockade in metastatic melanoma 

Presented by Crystal Mallow

For detailed scientific information on SEMA4D and pepinemab in oncology, please see our publications, presentations and posters.

Immunomodulation of the tumor microenvironment by neutralization of Semaphorin 4D

Evans, E.E. et al.

Invited “Author’s View”. OncoImmunology, 2015. 4:12

Corporate Presentation June 10, 2022

Pepinemab– Anti-SEMA4D Antibody for Cancer Immunotherapy

Presented by M. Zauderer, Ph.D.

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