Incorporation of Complex Membrane Proteins into Vaccinia
Vaccinex fusion protein technology provides for efficient incorporation of multi-pass membrane proteins into the vaccinia virus membrane. Untagged molecules do not incorporate efficiently.
![VacciniaLifeCycleGPCR](https://www.vaccinex.com/wp-content/uploads/2021/02/VacciniaLifeCycleGPCR.png)
- Mammalian Expression of ANY membrane protein:
- GPCRs, ion channels, multispanners and ECDs
- Expression Optimization by mutagenesis and selection
- One Size Fits All Purification of Proteins in their natural state
- Mammalian cell expressed protein contained in the viral membrane
- No protein optimization required
- No detergents or refolding
- Just Harvest the Virus
- 2 months from sequence to antigen
- Ideal for Antibody Discovery
- Suitable for vaccinia, phage, yeast displays and immunization
- Limited viral membrane complexity facilitates depletion of unwanted reactivities
Virions as Antigen for in vitro Antibody Selection
![Antigen-Virus-3-mobile](https://www.vaccinex.com/wp-content/uploads/2021/02/Antigen-Virus-3-mobile.jpg)
![Antigen-Virus-3](https://www.vaccinex.com/wp-content/uploads/2021/02/Antigen-Virus-3.jpg)
- Mini-library cloning into mammalian secreted IgG vector
- Transfect CHO
- Test by flow cytometry for binding to Antigen expressing cells